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Healthy ageing & energy

NAD+ Not a peptide

A molecule every cell uses for energy, discussed for ageing.

StatusNot an approved medicine for these uses; precursors sold as supplements in some markets
How well studied in peopleSmall human studies
Experts quoted5 experts

iNot medical advice. We don’t recommend any dose. Every dose below is a quote from the person named, describing their views or their own use. NAD+ is not a peptide. NAD+ precursors such as nicotinamide riboside are sold as food supplements in some markets. We found no EU, UK or US approval of injected or intravenous NAD+ for anti-ageing or wellness uses.

What experts say about dosing

4 statements from 3 people. Each one is word for word: we add punctuation only and mark cuts with […]. The chips repeat the exact words used.

Dr Craig KoniverPhysician; founder of Koniver Wellness (Charleston, US, and London)

With patients: 100 mg under the skin, five days on

Dose
“100 milligrams”
How often
“five days on, two days off”
How taken
“subcutaneously”

“Actually, that's worked out really well. We'll do 100 milligrams subcutaneously, again, five days on, take two days off. You get a little bit of that stomach cramping from the 100 milligram injection.”

Read the sourceDr. Craig Koniver: Peptide & Hormone Therapies for Health, Performance & Longevity (Huberman Lab transcript) · 7 Oct 2024

Context: What his clinics started doing during the pandemic. Before this he describes the in-clinic IV protocol they settled on: a 750 milligram “loading dose” given as five treatments in 10 days, then usually a monthly IV maintenance dose.

Vigorous SteveBodybuilding coach and YouTuber (VigorousSteve)

Own use: injections

Dose
“20 milligrams … or uh 40 milligrams split bilaterally”
How taken
“subq”

“So I, I just got sick of it. I just inject my glutathione intramuscularly three times a week 600 milligrams and NAD plus 20 milligrams subq or uh 40 milligrams split bilaterally as part of my interworkout protocol”

Watch at 50:44Nebido For Traveling, Skin-Friendly PEDs, GH Carpal Tunnel, Freeze Peptides, NAD+ Vs. NMN… (VigorousSteve Q&A, 28 Feb 2026) · 28 Feb 2026

Context: Explaining why he stopped going to IV clinics; said in a solo Q&A livestream.

Own use: subcutaneous instead of NMN

Dose
“20 milligs”
How often
“per day”
How taken
“subq”

“So I, I take it subq man. I stopped nicotine monucleotides because NAD plus 20 milligs per day is subq is way way way way cheaper than 800 to 1,000 milligrams NMN.”

Watch at 54:55Nebido For Traveling, Skin-Friendly PEDs, GH Carpal Tunnel, Freeze Peptides, NAD+ Vs. NMN… (VigorousSteve Q&A, 28 Feb 2026) · 28 Feb 2026

Context: Answering a viewer who asked whether subcutaneous NAD+ is worth it if IV is unaffordable.

Jay CampbellAuthor and podcaster on hormones and peptides; co-founder of a peptide company

Two to three days a week

Dose
“100mg”
How often
“2–3 days per week”
How long
“As needed”
How taken
“SubQ injection”
When
“AM”

“Dose: 100mg. Timing: AM. Frequency: 2–3 days per week. Duration: As needed. Administration: SubQ injection. […] Jay’s Note: NAD+ isn’t a skincare compound — it’s a cellular energy compound. But when your cells have the fuel they need to repair and replicate properly, your skin reflects that.”

Read the sourceJay Campbell’s Beauty, Skin & Rejuvenation Protocol (jaycampbell.com) · 30 Apr 2026

Context: Quoted from a protocol card on his website (the field labels Dose, Timing, Frequency, Duration and Administration are his). The protocol pages say every protocol has been “personally tested, refined, and used by Jay Campbell”, and most cards link to a product from BioLongevity Labs, a company he co-founded.

What experts say about safety

4 statements from 3 people about risks and how strong the evidence is.

Derek (More Plates More Dates)Fitness educator; founder of More Plates More Dates

Own use: precursor choice

“Personally, I use Nicotinamide Riboside (NR) because it is more cost effective right now, and it has human data to back up its efficacy and safety profile.”

Read the sourceDavid Sinclair Supplements For Anti-Aging – What He Takes & Why (moreplatesmoredates.com article) · 27 Jun 2019

Context: About NAD+ precursors (NR versus NMN), not NAD+ itself. Later in the article: “I take Resveratrol, a NAD+ precursor (NR as opposed to NMN), Vitamin K2 and Vitamin D.” No dose given.

Vigorous SteveBodybuilding coach and YouTuber (VigorousSteve)

Injection route and pro-oxidant effects

“um, well I think NAD plus should be done intervenous, uh, but I think you can do it subq, right […] now the problem is with injectable gluathione and N plus and other injectable antioxidants basically is that they might have some Pro, um, prooxidant effects at the site of administration”

Watch at 29:17IGF-1-LR3 Protocols, High-Dose Test… (VigorousSteve Q&A, 23 Dec 2023) · 23 Dec 2023

Context: Answering a viewer who asked whether subcutaneous NAD+ is worth it. Captions render NAD+ as “N plus” and glutathione as “gluathione”. The cut removes a remark about grey-market products.

Own experience: NR vs NMN

“Every time I took NR up to 1,200 milligs per day, I got a minute effect compared to a low dose of NMN. Now I've supplemented between 250 to 1,000 milligs of NMN per day. That's either with NAD+ administrations or without it.”

Watch at 1:31NAD+ & NMN: The Ultimate Energy And Mitochondrial Boost (VigorousClips) · 27 Oct 2025

Context: About oral precursors (NR, NMN), not NAD+ injections. His later video (above) says he has since stopped NMN in favour of subcutaneous NAD+.

Nicholas TrigiliHuman performance specialist and coach; fitness YouTuber

Side effects of higher doses

“And going higher doesn't get you more. It just gets you closer to more side effects, like the flushing of the face, the tightness in the chest”

Watch at 10:12The ONLY 2 Peptides You Need to Reverse Mitochondrial Decline in 2026 (At any age) (Nicholas Trigili) · 30 Jul 2026

Context: Said right after he talks about NAD+ dosing.

Go deeper

What the research used

Context, not a dosing guide. Human data on injected NAD+ are very limited. Most trials test oral precursors (NR, NMN) rather than NAD+ itself. None of this establishes an injection dose.

Human studies

StudyStudied inWhat the study usedWhat it found
Grant et al., 2019, Front Aging NeurosciPubMed 31572171Healthy men (small pilot)IV NAD+ at 3 µmol/min for 6 hoursNo change in plasma NAD+ for the first 2 hours; metabolites appeared in urine later
Christen et al., 2026, Nat MetabPubMed 4154025365 healthy adultsOral NR, NMN or nicotinamide for 14 daysNR and NMN raised blood NAD+ to a similar degree; nicotinamide did not
What we don’t know yet
  • NAD+ is a coenzyme, not a peptide.
  • Very few human studies of injected NAD+.
  • Flushing and chest tightness are mentioned by Nicholas Trigili as side effects of higher doses; we found no trial quantifying them.
  • Grey-market products are unregulated, so identity, purity and dose accuracy vary.
Common questions

Is NAD+ a peptide?

No. It is a coenzyme made from vitamin B3. We list it because it is often used in peptide stacks.

Does oral NR or NMN raise NAD+?

In a 2026 trial in 65 healthy adults, both raised blood NAD+ over 14 days.

Why does Nicholas Trigili’s dose say micrograms?

That is the unit he says, twice, in the video. We have not changed it.

Why does the protocol section only contain quotes?

We do not write our own dose ranges. The protocol cards show, word for word, what named people have publicly said, with their caveats and a link to the source.

About this page

Word-for-word statements about injectable NAD+ and NAD+ precursors, each with a link to the source. NAD+ is a coenzyme, not a peptide; it is included because it is often used alongside peptides. The research is listed separately as context.

Also known as
Nicotinamide adenine dinucleotide
Type
Coenzyme (not a peptide)
Last updated
11 Oct 2026

Last reviewed 11 Oct 2026 · Compiled by the Peptide Dosing editorial team · How we check quotes