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Expert profile

Bryan Johnson

Entrepreneur; Blueprint longevity project

4checked quotes
3on dosing
3peptides

Johnson publishes detailed accounts of his own longevity protocol and has posted about peptides he has used.

iNo endorsement. Bryan Johnson is quoted for public statements only and does not endorse this site. Quotes are word for word; follow each link for the full context.

BPC-157

Healing & recovery

On dosing

Personal protocol

Dose
“0.4mg of per shot”
How often
“1x daily”
How long
“20 days”
How taken
“per injection”
Preparation
“Reconstitute 10mg of BPC-157 in 5ml of BSW solution”

“My BPC-157 protocol: Reconstitute 10mg of BPC-157 in 5ml of BSW solution. Concentration will be 2mg/ml. Administer 0.20ml per injection, which provides 0.4mg of per shot. 1x daily for 20 days.”

Read the sourcePost on X (reply) · 10 Jun 2024

Context: A reply to a follower asking how he used it after his widely shared June 2024 ankle sprain post. It describes his own use only. Two years later he wrote (6 May 2026): "If you don't have the bandwidth for extensive testing, you are better off steering away from unregulated peptides."

On safety & evidence

Unregulated peptides

“If you don't have the bandwidth for extensive testing, you are better off steering away from unregulated peptides. The risk profile of research peptides like BPC-157, TB-500, CJC-1295, and ipamorelin is largely unknown.”

Read the sourcePost on X (reply) · 6 May 2026

Context: Opening of a longer post contrasting grey-market research peptides with approved peptide drugs, whose risk profile is characterised through three trial phases and post-marketing surveillance.

Melatonin

Calm, focus & sleep

On dosing

Own use: every night

Dose
“300 mcg”
How often
“each night”

“I take 300 mcg of melatonin each night, but otherwise don't take anything for sleep.”

Read the sourceBryan Johnson’s Evening Routine (blueprint.bryanjohnson.com) · 1 Jun 2026

Context: Item 6 of his published evening routine. Own use.

Cerebrolysin

Calm, focus & sleep

On dosing

Own use: daily for three months, no measurable effect

Dose
“5 mL”
How often
“daily”
How long
“three months”
How taken
“intramuscularly”

“Cerebrolysin Trialed daily 5 mL cerebrolysin injections intramuscularly for cognitive benefits for three months. No effect measured. This does not mean it didn’t help or hurt, we were just unable to identify a biomarker that could inform.”

Read the sourceBryan’s Protocol (protocol.bryanjohnson.com) · upload date not confirmed

Context: Own use, from the “Other Advanced Therapies” section of his protocol page. The page does not date the trial.

Sources used (4)