Nicholas Trigili

“So, it takes 3 to 6 months to actually notice any benefits or anything to start kicking in. The way I like to run this peptide is basically one to 2 milligrams subq daily or every other day for the best benefits consistently throughout the entire cycle is a must because it's going to produce the best results.”

“here's the exact protocol I would follow if I was starting over. Start conservative. You can always increase, but you can't undo taking too much. So, the dosing I would start with is 1 and 1/2 to 2 mg per day. The frequency, five times per week. The injection site, always subcutaneous, your abdominal wall or your upper thigh. You can rotate the sites to avoid the irritation. And the timing, morning, preferably fasted. And the consistency matters more than the timing. So, the cycle length I would do would be 60 to 90 days, then reassess based on your response and your results.”

“But here's how I ran it. One and a half to 2 milligram is the max dose per day five times per week subcutaneous. You could do your abdominal wall, your upper thighs. Ideally, just keep it consistent. Do in the morning first thing cuz consistent matters a lot. Minimum cycle, I would say 90 days. That's still pretty early before you're going to reassess anything. Again, start conservative. You can always go up.”

“I started my first retatrutide cycle around a half a milligram and every two weeks I started titrating my dose up. So I went from a half a milligram all the way up to four milligram even 6 milligram. From a half milligram I went to 2 milligram. That's where the best benefits actually showed up. My appetite came down but it was not zero. […] And this is the same pattern I'd seen with clients over and over and over. The best ones with the best results are the ones that actually stick to like the two to four milligram range. That's the range where you're actually still getting that metabolic and inflammatory benefits without completely destroying your ability to eat.”

“I followed that same principle though when I ran my own retatrutide experiment. I actually started much lower than the trials did. I started at a half a milligram and I eventually titrated up to 4 milligrams over the next six to seven weeks. […] You start at your lowest dose. You hold it for at least 2 weeks and you track your weekly loss. Now, if you're landing inside that 1 to 1.5% range and your side effects are still manageable, you do not touch that dose.”

“Let's say you peaked at 4 milligrams. You don't just stop cold, you taper. Weeks one and two, you drop from 4 milligrams down to 2 milligrams. Weeks three and four, you drop again to like 1 milligram. Week five, you drop to a half a milligram. That's roughly a six week taper from the decision that you decide to come off to your actual last injection.”

“let me walk you through exactly how I personally ran it and how I'd structure my protocol. I ran my first Epitalon cycle at 1 to 2 mg daily for 21 days. This was 5 years ago”

“I started conservative with my first cycle and I recommend you do the same if it's your very first time. This can run anywhere from 2 to 3 mg daily on the low end, and up to 5 to 10 mg sub-q daily, subcutaneous. And most people, like myself included, run it right before you go to bed. […] I don't stay on this continuously, and neither should you. I run it for 20 to 30 to 45-day cycles, and two to three times a year max.”

“I run this once or twice a year, 5 to 10 mg per day, subcutaneous, right before bed, because it works with your pineal gland and your circadian rhythm. It's very important. Max 30 days straight, minimum 21 days. Then completely off for 6 months before I even think about running it again.”

“The way I like to run MOTS-c is running it like this. I take 5 to 10 milligrams subq subcutaneously two to three times per week. Most people do well starting at 5 millig three times a week and adjusting it from there”

“And then, we have MOTS-c. I cycle for 12 to 16-week periods. […] I run MOTS-c at 5 to 10 mg per week. I split it into usually two doses.”

“I've been running MOTS-c on and off for 8 years. And this is what I would do if I were starting to run MOTS-c today. I would start with 1 mg five times a week. Typically, I like to do it first thing in the morning fasted or about 30 to 45 minutes before my training sessions. […] I would run that for 4 weeks before making any adjustments. If you're tolerating it well and you want to push a little bit further, you can move up to 2 mg five times a week. I would keep it there though for another 4 weeks.”

“SS-31 is typically dosed like this. I would start with 3 to 5 milligrams subq once a day. I like that my clients inject it first thing in the morning so it becomes part of their routine. they don't forget about it and they run it for a full length of your retatrutide cycle.”

“And SS-31, 5 to 10 mg per week, dose the same exact way. If I'm cycling together though, I start SS-31 by itself for the first two to four weeks to repair the machine. Then, you can layer MOTS-c on top of that.”

“So, here's how I would actually sequence this. I would start with SS-31 on its own. I would start with 2 mg five times per week for four weeks before I would start MOTS-c. Now, you can technically go two to four weeks.”

“So let's talk about the framework and the dosing with glutathione. Really simple 200 to 400 to even 600 milligrams injectable only. No tablets, no capsules, no troies, nothing. Two to three times per week minimum. Most men respond really well around 400 milligrams two times a week. […] if you drink, you smoke a lot, or you live a really stress intense life, you're going to need probably 800 milligrams a week.”

“When you're getting an IV of glutathione, it typically runs anywhere between 600 all the way up to 2,000 milligrams per session. Typically, it's once or twice a week. […] Now, if you'd rather skip the IV entirely, you have subq injections. Those are more practical and more consistent, easier to do. Typical dose, I would run a glutathione anywhere between 100 to 300 milligrams per day injected subq in the abdominal wall. […] Now, some people run it three, four, five times a week instead of daily, which still is going to maintain your levels just fine.”

“Typical dosing, 300 400 I mean, 600 micrograms is a lot. I don't typically go that high unless it's like I got to hammer it. Split into two doses, morning afternoon. You can cycle this a couple times a year. I don't recommend running as needed, but people do. […] I like structuring it to 21 to 30 days on and then off”

“And then there's Selank, which I always run with Semax. […] Same dosing as Selank and Semax, so they're 300 to 400 micrograms, maybe 600 if you're going crazy.”

“So, the dosing for this peptide typically runs between 8 to 10 mg a day over a 20-day cycle. I like to do 21 days, so 7 14 21, so it's 3 weeks, two to three times a year. Now, most people run this right after finishing a epitalon cycle. You can run them parallel to each other simultaneously.”

“I said no to the surgery. I took my chance on BPC at one to two maybe 3 milligs a day. Way too much. But nobody knew how to use this stuff properly back then. I injected as close I can to the injury site as I could. […] After a month though, I did drop down to 500 micrograms a day.”

“If I haven't used BBC157 in a while, I'm definitely going to start with a loading phase. And the loading phase is usually something very consistent like this, 21 to 30 days on daily, typically 1 mg a day. Yes, that's an aggressive loading phase. […] I will jump right to my maintenance phase, which is anywhere between 250 to 500 micrograms per day.”

“Here's how I actually structure these two today in my cycles. BPC-157. I run 500 micrograms a day. I start at the low end. I assess how my body responds for the first couple weeks. I look at my digestion. I look at my inflammation. I look at my recovery. And I adjust it from there. So just jumping straight into the high doses like I did that first time around. Not a good idea. If it's for a very specific injury, I'm injecting subcutaneous as close as possible. You can also do inter muscular. It's okay. It's not the end of the world. It's not going to affect anything negatively. If it's for general gut health or the systemic inflammation, subq in your abdominal wall works just as well. The minimal cycle length I suggest is at least 8 to 12 weeks.”

“And you could pair it with TB-500. And you're going to load anywhere between three to four, four to five milligrams twice per week. Never more than twice per week. Daily is a waste of this compound.”

“Now, TB-500 is ran a little bit different. TB-500 is only twice a week. So, I'll run 2.5 mg twice per week.”

“Now, TB-500 on the other hand doesn't need daily dosing. I run a loading phase for the first two weeks at about 5 milligrams twice a week to saturate your system really quickly. Then I drop to a maintenance phase for at least or I should say a week three or four up to 12 weeks and that's about five milligrams once a week.”

“Next up is KPV. KPV is going to target the gut inflammation at the immune level. Now, if you're dealing with severe bloating, IBS-like symptoms, eczema, or food tolerances, this shuts down that inflammatory cascade. You're going to add this in weeks 3 to 4. 500 to 1,000 micrograms daily. You're going to cycle off after 8 to 10 weeks once the inflammation normalizes.”

“PT-141 is dosed at typically 1 to 2 milligrams subq and it's used on an as needed basis, roughly 1 to two hours before you know you know what. For most of the clients that I work with, the sweet spot is two to three times per week throughout the cycle and as needed dose in layering on top of that when you actually want it. […] Start at the lower end though because PT-141 can cause a transient nausea and facial flushing.”

“Now you can inject these together always subcutaneously not interuscularly. And the best timing options are typically if you're going to use them both at the same time half that dose in the morning first thing in the morning when you get up half the dose right before bedtime. And the frequency, like I said, five days on, two days off. And the rest strategy, always try to take off two days in a row.”

“The dosing for these peptides CJC-1295 sits at about 200 to 400 micrograms combined with ipamorelin about 100 micrograms to 300 micrograms subcutaneous injection taken together right before bed 5 days a week is the best entry point into GH optimization on it entire list.”

“Sermorelin, you could start with anywhere between 250 to 500 micrograms daily. I've had guys go up to 1 milligram daily. […] You can inject them together subcutaneously. Now timing I always stick with something very similar just like I mentioned earlier with the five days on two days off approach.”

“Now, my experiments, don't copy these. I've used tesamorelin up to 2 milligrams a day, four to five to six times a week, never every day with these peptides. And I'm going to use this peptide for 16 to 30 to 40 weeks”

“Typically, you want to start with something like a half a milligram, even one milligram subcutaneous injection five days a week, administered before your bedtime to align with your body's natural GH pulse.”

“My favorite protocol, or I should say uses of IGF-1, would be typically around your workout. I like 30 minutes to 45 minutes pre-workout on your weight training day. Specifically, anywhere between 100 to 200 micrograms depending on where people's, let's say, IGF levels are or how they feeling, right? Could vary. And as far as the frequency goes, let's say you're training four or five times a week. That's fine. Definitely have one or two days off of IGF-1, but make sure you're monitoring your glucose levels because, well, hypoglycemia is real on this.”

“But layered on top of tesamorelin or CJC-1295 and ipamorelin it has a very specific lipolic signal that complements everything else running alongside it. The dosing is typically looking like 300 to 400 even 500 micrograms subq injected about 5 days a week ideally done in a fasted state in the morning to maximize the lipolic effect.”

“If you're just getting started, 500 to 1,000 IUs two to three times per week. That's it. If you're a more advanced user, you need higher stimulation based on your blood work, you're going to need to use 1,000 up to 4,000 IUs two to three times per week. This is all based on your response.”